Original Research Article
Year: 2018 | Month: October-December | Volume: 3 | Issue: 4 | Pages: 1-8
A Study of Lipid Peroxidation and Total Antioxidant Capacity in Hyperthyroid & Hypothyroid Female Subjects
Basant Joshi1, Sangeeta Singh2, Aman Saini3, Seema Gupta4, Vanishree B J5
1,2,3,4Department of Biochemistry, Govt. Medical College, Haldwani
5Department of Biochemistry, J. N. Medical College, Belgaum
Corresponding Author: Basant Joshi
ABSTRACT
Introduction: Thyroid hormones from the thyroid gland are necessary for the normal development of body organs. Variation in thyroid hormones (TH) is the most important factors involved in the regulation of the basal metabolic condition as well as in the oxidative metabolism. TH stimulates the production of free radicals and their disorders have pathogenic impact on human tissues. MDA, breakdown product of major chain reaction leads to definite oxidation of PUFA & serves as reliable marker of lipid per-oxidation (LPO). Total Antioxidant Capacity (TAC), instead of individual antioxidants, and LPO with changes in TH were seen in this study.
Materials & Methods: Serum T3, T4 & TSH by chemiluminescence assay, LPO by Okawa H et al (modified) and TAC by D Koracevic et al were estimated in 40 euthyroids, 20 hyperthyroids and 30 hypothyroid female subjects of age group of 25-50 years.
Results: The data was expressed as Mean±SD, statistical analysis performed using one-way ANOVA considering p<0.05 as lowest limit of significance. All these parameters differed significantly among the groups. The level of MDA was significantly higher in cases of hyperthyroid females when compared with euthyroid female subjects (p<0.01). No change was observed in hypothyroid females when compared with normal female subjects. The levels of TAC decreased significantly in both hyperthyroid and hypothyroid female patients when compared with euthyroid female subjects (p<0.01).
Conclusion: In conclusion, hyperthyroidism in females may cause LPO in tissues whereas hypothyroidism has no effects on LPO. The hyperthyroidism and hypothyroidism in females may cause changes in antioxidant defence system indicating increased tissue damage with alteration of thyroid hormones.
Key words: Hyperthyroid, Hypothyroid, Lipid Peroxidation, Total Antioxidant Capacity.