Original Research Article
Year: 2018 | Month: October-December | Volume: 3 | Issue: 4 | Pages: 52-58
Investigation of Thrombolytic and Antioxidant Potentials of Centella Asiatica
Md. Sakhawat Hossain1, Ratul Islam2, Sharmin Sultana3, Md. Hafizur Rahman4, Aditi Bhowmick3, Fatema-Tuz-Zohra3, Tushar Saha4
1Department of Pharmacy, Daffodil International University, Dhaka, Bangladesh.
2Department of Pharmacy, World University of Bangladesh, Dhanmondi, Dhaka, Bangladesh.
3Department of Pharmacy, University of Asia Pacific, 74/A, Green Road, Dhaka-1205, Bangladesh.
4Formulation Development, R&D, Square Pharmaceuticals Ltd., Dhaka, Bangladesh
Corresponding Author: Tushar Saha
The aim of the present investigation was to find out the antioxidant as well as the thrombolytic properties of Centella asiatica which is known as “Thankuni” in Bangladesh and used as a folk medicine. The methanolic extract of Centella asiatica plant was partitioned into petroleum ether soluble fraction(PSF), Chloroform soluble fraction (CSF), Ethyl acetate soluble fraction (ESF) and aqueous soluble fraction(ASF). The extracts were evaluated for their thrombolytic and antioxidant potentials and the results were compared with standard drugs; streptokinase for thrombolytic. In thrombolytic investigation, the aqueous soluble fraction (ASF) showed highest percent of clot lysis (50.53%) as compared to (63.74%) exhibited by the standard streptokinase. The presence of antioxidant potentials of Centella asiatica were evaluated by antioxidant capacity and reducing power to free radical. The total antioxidant capacity of Methanol extract of Centella asiatica (MCA), PSF, CSF, ESF and ASF were observed as mg of extract, respectively. The DPPH free radical in term of IC50 values of the extractives was found 108.25 µg/ml, 909.03 µg/ml, 292.44 µg/ml, 104.61 µg/ml and 173.09 µg/ml for MCA, PSF, CSF, ESF and ASF respectively in comparison to 400.02 µg/ml for BHT. The result obtained in this study found that Centella asiatica plant possesses potential thrombolytic and antioxidant effect.
Key words: Centella asiatica, thankuni, antioxidant, thrombolytic effect.