Original Research Article
Year: 2019 | Month: January-March | Volume: 4 | Issue: 1 | Pages: 16-23
Progesterone Regulates Superoxide Dismutase, Catalase, Malondialdehyde and Cholinesterase Activities in Trimethyltin-Induced Hippocampal Damage in Adult Male Wistar Rats
Okesina A.A1, Ajao M.S2, Tagoe C.N.B3, Mesole S. B4, Bello T.O5, Okeniran O.S6, Olalekan O.E7
1Lecturer, Department of Anatomy, Faculty of Medicine and Surgery, University of Gitwe, P.O. Box 01, Nyanza, Rwanda.
2Associate Professor, Department of Anatomy, College of Health Sciences, University of Ilorin, PMB 1515, Ilorin, Nigeria.
3Professor, Department of Anatomy, College of Health Sciences, University of Ghana,
4Department of Anatomy, Faculty of Medicine and Surgery, University of Gitwe, P.O. Box 01, Nyanza, Rwanda, Lecturer.
5Lecturer, Department of Biochemistry, Faculty of Medicine and Surgery, University of Gitwe, P.O. Box 01, Nyanza, Rwanda,
6Tutorial Assistant, Department of Anatomy, Faculty of Medicine and Surgery, University of Gitwe, P.O. Box 01, Nyanza, Rwanda,
7Lecturer, Department of Internal Medicine, Faculty of Medicine and Surgery, University of Gitwe, P.O. Box 01, Nyanza, Rwanda,
Corresponding Author: Okesina A.A
ABSTRACT
Background: Reactive oxygen species (ROS), also known as free radicals, are normal by-products of mitochondrial respiratory chain activity. Overproduction of these free radicals can cause oxidative damage to bio-molecules, (lipids, proteins, DNA), which eventually can lead to chronic diseases like stroke, aging and other neurodegenerative diseases.
Objective: Hence, this study was designed to investigate the effect of progesterone (PROG) on antioxidant biomarkers such as superoxide dismutase (SOD), catalase (CAT) and malondialdehyde (MDA) as well as a biomarker in environmental study, cholinesterase (CHOL); following trimethyltin (TMT) induced neurotoxicity in the hippocampus of adult male Wistar rats.
Methods: Twenty four adult male Wistar rats were used in the study and divided into three groups. Group A as positive control was given 0.2 ml of normal saline (NS), group B, as negative control was administered 8 mg/kg (TMT) start dose only, while group C was administered 8mg/kg TMT start and 16 mg/kg of progesterone (TMT-PROG). All treatments were done intraperitoneally. The brains were excised and homogenized for enzyme analysis.
Results: The result of this study revealed that, in the TMT treated animals, SOD and CAT activities were significantly reduced while MDA, CHOL levels were significantly increased as compared to the animals that received only normal saline. In the TMT-PROG group, the activities of SOD and CAT were significantly increased while the activity of CHOL and MDA quantity were significantly reduced as compared to TMT-treated group. Values are presented as mean±SEM (*P<0.05).
Conclusion: After the biochemical analysis of the hippocampus of adult male Wistar rats, using superoxide dismutase, catalase, malondialdehyde, and cholinesterase to study oxidative, the study revealed that progesterone (16mg/kg) reduced the adverse effect caused in the hippocampus by trimethyltin (8mg/kg).
Key words: Trimethyltin, Reactive oxygen species, Superoxide dismutase, Catalase, Malondialdehyde, Cholinesterase, Progesterone.