Original Research Article
Year: 2019 | Month: July-September | Volume: 4 | Issue: 3 | Pages: 66-70
Correlation of Oxidative Stress with Lipoprotein (a) In Type 2 Diabetes Mellitus
Shubhangi M. Dalvi1, Chandrakiran D. Hathial2, Neelam Yeram3, Mayuri Nalavade4, Vinayak W. Patil5
1Associate Professor, Department of Biochemistry, Grant Government Medical College and Sir J. J. Group of Hospitals, Mumbai 400008.
2Associate Professor, Department of Physiology, Grant Government Medical College and Sir J. J. Group of Hospitals, Mumbai 400008.
3Research Scholar, Department of Biochemistry, Grant Government Medical College and Sir J. J. Group of Hospitals, Mumbai 400008.
4Ex-M.Sc. Student, Department of Biochemistry, K.J Somaiya College of Science & Commerce, Vidyavihar, Mumbai 400077.
5Ex-Professor and Head, Department of Biochemistry, Grant Government Medical College and Sir J. J. Group of Hospitals, Mumbai 400008.
ABSTRACT
Background: Diabetes mellitus is a group of metabolic diseases characterized by hyperglycemia resulting from defects in insulin secretion and insulin action or both. The chronic hyperglycemia is associated with long-term damage, dysfunction, and failure of normal functioning of various organs. Hyperglycaemia generates reactive oxygen species, which in turn cause damage to cells, ultimately resulting in secondary complications. Diabetic patients have a high risk of cardiovascular disease. Lipoprotein (a) is identified as a major risk factor of atherosclerosis in non-diabetic and diabetic patients.
Objective: In this study, we have analysed the serum levels of MDA, NO and lipoprotein(a) in newly diagnosed type 2 diabetes patients.
Methods: Case-control study comprising of aged-sex matched subjects: newly diagnosed T2DM cases (n=30) and controls (n=30). The serum samples of subjects were analysed for levels of MDA by Buege and Aust method, while NO levels by Cortas and Wakid’s kinetic cadmium reduction method using spectrophotometer. Lipoprotein (a) levels were analysed using agarose gel electrophoresis. Statistical analysis was done using Mini-tab 17 software with 95% confidence interval.
Results: Serum levels of MDA and NO in T2DM patients were significantly increased as compared to healthy controls. There was no significant difference in lipoprotein(a) levels in diabetic patients.
Conclusion: There is increased oxidative stress in type 2 diabetes which affects the functioning of other body organs causing disease progression. Antioxidant supplements might help control and keep check on disease status.
Key words: Newly diagnosed Type 2 Diabetes Mellitus, MDA, Nitric Oxide, Lipoprotein (a).