Original Research Article
Year: 2019 | Month: July-September | Volume: 4 | Issue: 3 | Pages: 137-141
Sensitivity Pattern of Levofloxacin and Nitrofurantoin in Patients of Urinary Tract Infection in a Tertiary Care Hospital, Jaipur
Ayushi Sharma1, Dr. Mohit Agrawal2, Dr. Ved Prakash Mamoria3
1Department of Microbiology, Mahatma Gandhi University of Medical Sciences and Technology, Jaipur
2Associate Professor, 3Professor and Head,
Department of Microbiology, Mahatma Gandhi University of Medical Sciences and Technology, Jaipur
Corresponding Author: Dr. Mohit Agrawal
ABSTRACT
Background: There has been upsurge in the antibiotic resistance mechanisms in the uropathogens over the years. Fluoroquinolones have gained popularity over time and are being used to treat a number of infections. This study was undertaken to assess the distribution patterns of uropathogens and their sensitivity towards levofloxacin and nitrofurantoin.
Method: A total of 628 urinary pathogens were isolated from the month of June 2018 to December 2018. A semi quantitative method was taken on for the primary isolation of organisms using a calibrated loop which delivers 0.01 ml of urine onto Blood agar and MacConkey agar media plates. The plates were then incubated at 37℃ overnight. Samples with a colony count of ≥105cfu/ ml were considered with significant bacteriuria. The final identification of the organism and its antibiotic susceptibility testing was done on Vitek 2 Compact™ (Biomerieux, France).
Result: In this study we isolated 73.5% (462) Gram Negative Bacilli, while 13.69% (86) were Gram Positive Cocci isolates and 12.7% (80) were Candida isolates. E. coli was the predominantly isolated organism (48.40%) followed by Klebsiella pneumoniae (10.82%) and Enterococcus faecalis (5.73%). Levofloxacin was found to be 20.98% sensitive for Gram Negative Bacilli while it was sensitive for 20.93% of the Gram Positive Cocci isolates. Nitrofurantoin showed a sensitivity rate of 65.70% for Gram Negative Bacilli and 72.09% for Gram Positive Cocci isolates.
Conclusion: Increasing prescription of fluoroquinolones have resulted in the drugs like levofloxacin and nitrofurantoin losing their effectiveness. The data in this study shows high resistance pattern to second generation fluoroquinolones and increasing resistance to nitrofurantoin.
Key words: Fluoroquinolones, levofloxacin, nitrofurantoin, Uropathogens, E.coli