Original Research Article
Year: 2019 | Month: July-September | Volume: 4 | Issue: 3 | Pages: 35-43
Bacteriological Isolates and Their Phenotypic Expression of Enzyme Related Resistance Observed From a Tertiary Care Hospital: A Two Year Study
Dr Shalabh Jauhari1, Dr Sulekha Nautiyal2
1,2Associate Professor, Dept of Microbiology & Immunology, Shri Guru Ram Rai Institute of Medical & Health Sciences, Shri Mahant Indiresh Hospital, Dehradun, Uttarakhand.
Corresponding Author: Dr Sulekha NautiyalABSTRACT
Purpose: This study was designed with the objective of finding the incidence of bacteriological infections over a period of 2 years and to find out their association with enzyme related resistance in our hospital settings.
Methods: This was a retrospective study from Jan 2016 to Dec 2017. Of the total 35,800 specimens; Blood (8770), Urine (14749), Pus (3621), all types of tips (8660) were studied. Aerobic culture followed by automated identification and antimicrobial susceptibility of pathogens were done.
Results: Aerobic culture of blood, pus, urine & all types of tips showed culture positivity of 12.94%, 55.43%, 21.17% & 10.87% respectively. Predominant isolates from these specimens were Acinetobacter spp, Enterobacter spp, Escherichia coli, Klebsiella spp, Proteae family, Pseudomonas spp, Staphylococcus aureus, Enterococcus spp accounting for12.31%, 4.07%, 36.61%, 13.99%, 3.63%, 10.48%, 5.52% & 6.66% respectively. Staphylococcus aureus was found to be mecA & heteroVISA positive in 45.94% & 18.53% respectively. Enterococcus spp expressed Van A like and Van B like resistance in 27.35% and 6.47% respectively.
Conclusions: It is important for every hospital to have a data on prevalent organisms and their antibiotic susceptibility pattern.
Key words: Antimicrobial drug resistance, Carbapenemase, ESBL, heteroVISA, mecA.