Original Research Article
Year: 2020 | Month: January-March | Volume: 5 | Issue: 1 | Pages: 6-12
Role of Endothelial Nitric Oxide Synthase (T-786C) Gene Polymorphism in the Development of Coronary Artery Disease
Namrata Bhutani1, Deepak Tangadi1, Prabhash Bhavsar2
1Senior Resident, Department Of Biochemistry, Vardhaman Mahavir Medical College, New Delhi,
2Assistant Professor, Government Medical College, Dungarpur.
Corresponding Author: Deepak Tangadi
ABSTRACT
Background: Recent studies suggest a cause and effect relationship between eNOS gene polymorphism and increase incidence of insulin resistance as NO deficiency is associated with decreased vasodilation and altered binding of insulin to insulin receptor.
Aim: This study aimed to study eNOS -786T/C gene polymorphism in patients with CAD in Indian population.
Methodology: This study consisted of 60 adult patients of with documented CAD and 60 age and sex matched healthy subjects as controls. Fasting Serum Insulin was measured by ELISA, fasting Serum nitric oxide by modified Griess reaction and fasting plasma glucose on fully automated chemistry analyzer (Hitachi 902) HOMA-IR was calculated mathematically by using formula given by Matthew et al. The eNOS gene loci was amplified by using PCR and by RFLP. A p-value <0.05 was considered significant. Statistical analysis was performed with the help of SPSS version.
Results: The mean serum NO levels were very significantly lower in case groups and mean HOMA-IR levels in the study group were very significantly higher as compared to the control group (p=0.000) suggesting the role of insulin resistance in CAD. The frequency of T allele was 76.67% in the study group and 81.67% in the control group while the frequency of C allele was 23.33% in the study group 18.33% in the control group respectively. This difference was found to be statistically significant .(p=0.0123)
Conclusions: Endothelial Nitric Oxide Synthase (T786C) gene polymorphism, is a significant risk factor for development of CAD.
Keywords: Endothelial nitric oxide synthase (T-786C), gene polymorphism, Coronary artery disease.