Original Research Article
Year: 2018 | Month: September | Volume: 3 | Issue: 3 | Pages: 43-46
Comparative Evaluation of Electrochemiluminescence (ECLIA) with Immunochromatographic Test (ICT) Available for Hepatitis B Surface Antigen
Dr. Sarika Ghai1, Dr. Payal Dutta2
1Assistant Professor, Department of Microbiology, ASCOMS and BATRA Hospital, Sidhra Jammu, J&K, 180004.
2Senior Resident/Tutor/Senior Demonstrator, Department of Microbiology, ASCOMS and BATRA Hospital, Sidhra Jammu, J&K, 180004.
Corresponding Author: Dr. Payal Dutta
ABSTRACT
Introduction: Hepatitis B virus infection is a major global health problem with predilection for liver and is known to commonly lead to chronic infections. Purpose of this study is to detect the presence of hepatitis B surface antigen (HBsAg) by ICT and ECLIA, compare them and to check the accuracy of ECLIA.
Material and methods: A study was conducted over the period of 9 months on serum samples for the detection of Hepatitis B surface antigen by ICT method and ECLIA. Results: 1020 serum samples were tested. Among these, 30 were found to be reactive. Out of these 30 positive samples, 18 were reactive with both ECLIA and ICT and had COI >32 where as 12 were reactive with ECLIA alone and had COI between 0.9-32. Among these 12, 5 samples had COI between 0.9-32 and were considered as Weakly Reactive. However, 7 samples had COI between 0.9-1 and were taken as Borderline reactive.
Conclusion: In our study, ECLIA is an accurate and good test method for the detection of HBsAg in the patients.
Key words: HBsAg, ECLIA, Immunochromatographic ICT.