Review Article
Year: 2020 | Month: July-September | Volume: 5 | Issue: 3 | Pages: 38-46
Development and Analysis of Analytical Methods for Determination of Catechins and Quercetin in Natural Products: A Review
Sherly Emilia, Rina Desni Yetti, Ridho Asra
College of Pharmacy (STIFARM), Jl. Raya Siteba Kurao Pagang, Padang, West Sumatera, Indonesia
Corresponding Author: Rina Desni Yetti
ABSTRACT
Catechins are polyphenolic compounds that have the potential as an antioxidant and antibacterial as well as safe for use in food processing, and the physicochemical properties of the catechins may be a drug dosage formulations. Catechins can be used for pharmaceutical preparations. Catechins are a group of secondary metabolites that are naturally produced by plants and are included in the flavonoid group while quercetin is a flavonoid compound that was found in many vegetables and fruits. In addition to having a very strong antioxidant activity, quercetin also has other biological activities such as antiviral, antibacterial, anti-inflammatory, anticancer, and also can serve as a hypo-allergenic, and hypertension. In addition, quercetin was also used for a number of pharmacological activities such as diabetes and wound healing drugs. Several studies have shown that quercetin has significant activity in inhibiting some cancer cells such as breast, prostate, colon, and lung. Catechins and quercetin has been widely used in Indonesia for treatment. Over the years, a variety of analytical methods have been developed.This review focuses on the latest developments in analytical techniques to analyze catechin and quercetin contained in the products of natural materials. This review provides some insight into the analytic methods, UV-Vis Spectrophotometry, High Performance Liquid Chromatography (HPLC), Mass Spectrometry High Performance Liquid Chromatography (HPLC-MS), Liquid Chromatography Vacuum (VLC) using a gradient elution and Thin Layer Chromatography (TLC). The presence of catechin compounds in each fraction of the VLC process was monitored by TLC. Catechin levels were determined by the UV-Vis spectrophotometer. Based on the results of the UV-Vis spectrophotometer, the levels of natural ingredients were adjusted to international standards. The results of Liquid Chromatography Mass spectrometry (LC-MS) showed that natural materials were analyzed which contains catechins and quercetin.
Keywords: Catechins, quercetin, analytical techniques, natural ingredients.