Year: 2023 | Month: October-December | Volume: 8 | Issue: 4 | Pages: 85-90
DOI: https://doi.org/10.52403/gijhsr.20230411
Antibacterial Irrigation Solution Cocoa Bean Cuticle Extract (Theobroma cacao L) Against Streptococcus viridans
Dwi Warna Aju Fatmawati1, Rahmat Agung2, Raditya Nugroho3
1,3Conservative Department, Faculty of Dentistry, University of Jember, Indonesia
2Faculty of Dentistry, University of Jember, Indonesia
Corresponding Author: Dwi Warna Aju Fatmawati
ABSTRACT
Background: Pulp necrosis is the demise of the pulp due to persistent infection in the pulp area, such as Streptococcus viridans. Irrigation steps during biomechanical preparation and sterilization play a very important role. The irrigation material often used is 2.5% NaOCl because it has a broad antibacterial spectrum but is irritating and has an unpleasant aroma. The cuticle of cocoa beans (Theobroma cacao L.) might be an alternative root canal irrigation material.
The Objective: To determine the antibacterial properties of irrigation solution of cocoa bean cuticle extract against Streptococcus viridans.
Materials and Methods: This study was laboratory experimental with a post-test-only control group design by calculating the number of bacterial colonies. Consisting of eight research groups, namely cocoa bean cuticle extract with concentrations of 25% (P1), 12.5% (P2), 6.25% (P3), 3.12% (P4), 1.56% (P6), 0.78% (P6), NaOCl 2.5% (K+), and sterile distilled water. 0.5 ml of each group was taken and then mixed with 0.5 suspension of Streptococcus viridans, diluted 10-4. Next, the extract and bacterial suspension mixture 10-4 were incubated for 24 hours, then 0.1 ml was inoculated on BHIA media and incubated. After that, the colony counter manually counted the number of colonies.
Results: The result showed a significance of p<0.5, followed by the Mann-Whitney test, which showed significant differences except in group P1 (25%) with K+ (NaOCl 2.5%).
Conclusion: Cocoa bean cuticle extract irrigation solutions with concentrations of 25%, 12.5%, 6.25%, 3.12%, 1.56% and 0.78% presented antibacterial power against Streptococcus viridans.
Keywords: Streptococcus viridans, Antibacterial, Cocoa bean epidermis extract, Irrigation solution.